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Moredun Proteomic Facility Services

MPF staff are pleased to contribute towards experimental design, protocol development, data analysis, etc. Complete CONFIDENTIALITY can be provided where required.

Contact us to discuss your specific requirements.

Where MPF staff members contribute towards your research, recognition through co-authorship and/or acknowledgement of MPF and its staff is required.  Click here for Guidelines. 

Services Available

Pre-Fractionation of Complex Protein Mixtures

  • HPLC (nano-flow; capillary-flow; microbore; analytical)
  • Liquid IEF
  • Solid Phase Extraction
  • Gel Electrophoresis

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An important initial step in any proteomics workflow is pre-fractionation of complex protein mixtures, e.g. crude cell lysates, into manageable simplified samples that facilitate high resolution analysis by subsequent 2-D gel electrophoresis and/or mass spectrometry. This can be achieved in a number of ways including liquid iso-electric focusing (liquid IEF) and more traditional single and multi-dimensional chromatographic (HPLC) strategies.

2-D Gel Electrophoresis

  • IPGPhor™ IEF (1st dimension)
  • MultiPhor™ and Ettan-DALT™ (2nd dimension) platforms
  • Gel formats for pilot (7cm) and analytical (up to 24cm) studies

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The Moredun Proteomics Facility is equipped with a number of GE Healthcare IPGPhor™ IEF (1st dimension) and MultiPhor™ and Ettan-DALT™ (2nd dimension) platforms. A wide selection of 1st and 2nd dimension gel formats is available ranging from smaller (7cm) gels for pilot experiments to large (24cm) gels for more definitive high-resolution work.

Liquid Chromatography

  • Dionex UltiMate™ and BioLC
  • Beckman-Coulter System-Gold HPLC
  • Size exclusion, ion exchange, hydrophobic interaction and reversed phase separations
  • Multi-dimensional chromatographic purification strategies

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The Moredun Proteomics Facility can provide chromatographic separations on analytical, micro-bore, capillary-flow and nano-flow scales. Dionex UltiMate™ and Beckman-Coulter System-Gold HPLC platforms provide for size exclusion, ion exchange, hydrophobic interaction and reversed phase separations for a range of preparative and analytical applications. Single- and multi-dimensional chromatographic protocols can be custom-tailored to yield highly enriched target molecules.

Mass Spectrometry

  • Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-ToF MS/MS)
    • Bruker UltraflexII
  • Liquid Chromatography Electrospray ionisation MS/MS
    •  Bruker amaZon-ETD spherical ion trap
    •  Bruker maXis Ultra-High-Resolution Q-ToF

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The Moredun Proteomics Facility is equipped with three tandem mass spectrometers including a Bruker Ultraflex II MALDI-ToF, a Bruker maXis UHR-Q-ToF and a Bruker amaZon-ETD 3-dimensional ion trap. Collectively, these instruments enable the MPF to provide a comprehensive range of MS applications including protein identification, de novo characterisation of peptides, analysis of post-translational modifications and label-free quantitation.

Applications

  • Protein identification
  • Shotgun proteome analysis
  • Label-free Quantitation
  • Protein characterisation (including Post-translational modifications)
  • T3 Protein Sequencing
  • Whole cell MALDI (microbial identification & differentiation)
  • Top-down proteomics (ETD & PTR)
  • Intact Mass Analysis
  • LC-MALDI
  • Biomarker Identification & Characterisation (ClinProt; SELDI)

Analytical Tools

  • MASCOT
  • ProteinScape

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Printed from http://www.moredun.org.uk/commercial-work/proteomics/moredun-proteomic-facility-services on 25/06/17 01:13:14 PM

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