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Chlamydia Genome Sequencing Project

As part of a multicollaborative project funded by the BBSRC (BB/E018939/1), as part of the Combating Endemic Diseases of Farmed Animal Species initiative, to investigate the heterogeneity in carriage and expression of a novel group of autotransporter proteins of chlamydia species, a large number of strains of different geographical origin (Europe wide) and host animal species have been collected to examine diversity.  These are representative strains of the two major pathogens of livestock, causing abortion (Chlamydia abortus) and a range of disease syndromes, including infertility, pneumonia, encephalomyelitis, metritis, enteritis, mastitis, conjunctivitis and polyarthritis (Chlamydia pecorum) in sheep, cattle and pigs.  These strains are being sequenced using second generation technologies (Illumina) in collaboration with Professor Nick Thomson and Dr Helena Seth-Smith of the Wellcome Trust Sanger Institute,  

comparision 2The resulting genome sequencing will allow an insight into the variability of chlamydial field strains with reference to host species and geographical location, and will provide important information to help in our understanding of how these pathogens differ in their ability to induce disease. Ultimately this will help in the development of improved disease control strategies.

We therefore would like to invite any researchers who have C. abortus and/or C. pecorum strains in their pathogen collections, or who are able to isolate the organisms from clinical samples, to submit them to us at Moredun for sequencing. At present there is no end date for receipt of samples, please send them to us as and when you are able to grow the organisms for the preparation of genomic DNA. A minimum of 5 μg (preferably 5-10 μg) of purified gDNA at a concentration of 100 ng/μl is required.

Comparison 1

Click here for a downloadable excel spreadsheet, which details the information required for each isolate, which should be submitted to us in advance of submission of any gDNA samples. Please provide as much information as you can for each strain, but do not worry if the information is limited or incomplete.

Click here for a pdf of a suggested method for the purification of gDNA from egg or cell culture grown material.

Click here for a pdf listing the strains that have been committed and gDNA provided to the project. This file will be frequently updated to also include information on sequencing status.

Click here for a mailing label for sending gDNA samples to us here at Moredun.

If you have any questions or require further information please contact Dr David Longbottom.

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Printed from http://www.moredun.org.uk/research/research-%40-moredun/reproductive-diseases/chlamydia/genome-project on 23/05/17 02:00:10 AM

Moredun is committed to promoting animal health and welfare through research and education and is recognized worldwide for its contribution to research into infectious diseases of farmed livestock.