Kevin McLean

Kevin is the manager of the Proteomics Facility at Moredun Research Institute. The facility offers a range Proteomic analysis services to Moredun researchers and external collaborators. The services provide researchers with a comprehensive suite of tools for the investigation of infectious diseases and facilitate the identification of vaccine candidates and development of disease control measures.

The facility currently operates three mass spectrometers:

• Bruker Ultraflex II MALDI-ToF, primarily used for Peptide Mass Fingerprinting (PMF), intact protein analysis and Whole Cell MALDI.
• Bruker amaZon-ETD ion trap, used for identification of proteins from complex mixtures, characterisation of Post-Translational Modifications and Gel-LC analysis.
• Thermo Scientific Q-Exactive Plus Orbitrap, this recently acquired High Resolution instrument offers resolution up to 140000 for identification and quantitative analysis of complex protein samples.

Kevin’s areas of focus are protein identification and quantification, and developing informatics techniques for analysing mass spectrometry data outputs. Kevin has worked extensively in developing Whole Cell MALDI analytical workflows and post-data acquisition analysis pipelines utilising MALDI data to differentiate between closely related bacterial isolates.